论文部分内容阅读
用DEAE-纤维素层析和羟基磷灰石层析方法,从牛心中提取了cAMP-依赖性蛋白激酶。经SDS-PAGE电泳鉴定分子量为38kDa,并测定了其活性,为cAMP代谢途径的研究打下了基础。
CAMP-dependent protein kinases were extracted from bovine hearts using DEAE-cellulose chromatography and hydroxyapatite chromatography. The molecular weight of 38kDa was determined by SDS-PAGE electrophoresis and its activity was determined, laying a foundation for the study of cAMP metabolic pathway.