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应用ＰＣＲ方法研究了维甲酸（ＲＡ）和二甲基亚砜（ＤＭＳＯ）诱导ＨＬ－６０细胞分化过程中ｃ－ｍｙｃ基因扩增的变化。结果：ＨＬ－６０细胞内ｃ－ｍｙｃ基因扩增为１１，经ＲＡ诱导６ｄ后，其扩增程度无明显变化，而ＤＭＳＯ诱导３ｄ后，ｃ－ｍｙｃ基因扩增出现下降，至５ｄ，较未诱导分化前下降约３０％。提示了ＲＡ和ＤＭＳＯ对ＨＬ－６０细胞不同的诱导分化机制。
The PCR method was used to study the changes of c-myc gene amplification in the differentiation of HL-60 cells induced by retinoic acid (RA) and dimethyl sulfoxide (DMSO). RESULTS: The amplification of c-myc gene in HL-60 cells was 11. After 6 days of RA, there was no significant change in the amplification of c-myc gene. However, after 3 days of DMSO induction, c-myc gene amplification decreased to 5 days. Decreased by about 30% before induction of differentiation. The different induction mechanisms of differentiation of HL-60 cells by RA and DMSO were suggested.