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以一个耐盐的二倍体野生种旱地棉和对盐敏感的陆地棉栽培种苏棉12号为材料,运用cDNA-AFLP技术,比较两个材料分别在盐胁迫前后的表达情况,获得了25个仅在旱地棉盐胁迫下特异表达的转录片段(TDF)。将这些片段进行电子克隆,延伸后的序列进行BLAST分析,结果显示23个转录片段推断的氨基酸序列与已知的蛋白同源,这些盐诱导表达的基因主要涉及离子转运、活性氧清除、细胞信号传导、细胞分裂、转录调节、膜保护、渗透调节等功能蛋白。从23个差异表达的转录片段中选择9个进行实时定量PCR(qRT-PCR)分析,结果表明这些基因在盐胁迫后表达显著增强,而且多数在12~24 h达到高峰。这些cDNA克隆是开展棉花耐盐性分子基础研究的重要资源。
A salt-tolerant wild-type dryland cotton and saline-tolerant cotton cultivars Sumian 12 were used as materials to compare the expression of the two materials before and after salt stress with cDNA-AFLP technique. The results showed that 25 A transcript fragment (TDF) that is specifically expressed only under salt stress in upland cotton. The results of BLAST analysis showed that the deduced amino acid sequences of 23 transcripts were homologous with known proteins. The genes induced by these salts mainly involved in ion transport, reactive oxygen species scavenging, cell signaling Conduction, cell division, transcription regulation, membrane protection, osmotic adjustment and other functional proteins. Nine of 23 differentially expressed transcripts were selected for qRT-PCR analysis. The results showed that these genes were significantly enhanced after salt stress, and most of them reached the peak at 12-24 h. These cDNA clones are important resources for the basic research on the molecular basis of salt tolerance in cotton.