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目的：探讨实验性肝细胞增生酶异常灶量化指标的检测方法。方法：应用抑制物抑制二乙基亚硝胺（ＮＤＥＡ）致大鼠肝细胞增生酶异常灶（γ－谷氨酰转肽酶阳性灶和胎盘型谷胱甘肽ｓ－转移酶阳性灶）发生的短期实验模型以及电脑化图形扫描分析系统对肝细胞增生酶异常灶进行测量，统计比较其结果。结果：三个肝叶切片的总值和任一肝叶切片数值以及各肝叶切片数值之间比较差异不显著；评价抑制物抑制γ－谷氨酰转肽酶（γ－ＧＴ）灶发生情况时，以右前叶数值更接近三叶总值；不同人员处理相同的蜡块，所得结果在单位面积的灶数（个／ｍｍ２）、灶面积密度（ｍｍ２／ｃｍ２）上相比差异不显著；γ－ＧＴ与胎盘型谷胱甘肽Ｓ－转移酶（ＧＳＴＰ）染色结果比较差异显著，ＧＳＴＰ数值大于γ－ＧＴ。结论：评价肝细胞增生酶异常灶的发生时，以右前叶代表性最强；我们的观察方法和所得的结果重复性好；ＧＳＴＰ染色较γ－ＧＴ染色更准确并可节省工作量，是一种较优越的染色方法。提示研究大鼠肝细胞增生酶异常灶时，选择右前叶作为检测对象，ＧＳＴＰ作为阳性标志酶更简便可行。
Objective: To explore the detection method of quantitative indexes of experimental hepatocyte proliferation enzyme abnormalities. METHODS: Inhibitors were used to inhibit diethylnitrosamine (NDEA)-induced hepatocyte proliferation enzyme abnormalities (γ-glutamyl transpeptidase positive lesions and placental glutathione s-transferase positive lesions) The short-term experimental model and computerized graphic scanning analysis system were used to measure abnormal hepatocyte proliferation enzyme and statistically compare the results. Results: There were no significant differences in the total values of the three liver lobes and the values of any liver lobe slice and the number of liver lobe slices; the inhibitors were evaluated for the inhibition of the occurrence of γ-glutamyl transpeptidase (γ-GT) foci At the same time, the value of the right anterior lobe is closer to the total value of the trifoliate; different people deal with the same wax block, the results obtained are not significantly different in the number of foci (units/mm2) and area density (mm2/cm2) per unit area; There were significant differences between γ-GT and placental glutathione S-transferase (GSTP) staining. The GSTP value was greater than that of γ-GT. Conclusion: When assessing the occurrence of hepatocyte proliferation enzyme abnormalities, the right anterior leaf is the most representative; our observation method and the results obtained are reproducible; GSTP staining is more accurate than γ-GT staining and can save workload. A superior dyeing method. When studying abnormal hepatocyte cell proliferation in rats, the right anterior leaflet was selected as the test object, and GSTP as a positive marker enzyme was more convenient and feasible.