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动态观察ＨＢＶ与ＡＦＢ１诱发树肝细胞癌（ＨＣＣ）病变过程中肝癌前病变γ－谷氨酰转肽酶阳性肝细胞灶（ＧＧＴ阳性灶）、胰岛素样生长因子Ⅱ（ⅠＧＦ－Ⅱ）发生情况，探讨ＨＣＣ发生的可能机制。实验动物分４组，Ａ组：ＨＢＶ阳性和摄入ＡＦＢ１；Ｂ组：ＨＢＶ阳性组；Ｃ组：摄入ＡＦＢ１；Ｄ组：空白对照。组织化学及免疫组织化学检测肝活检组织。结果：（１）肝癌前病变ＧＧＴ阳性灶在各实验组不仅灶数量，而且灶大小均明显高于空白组（Ｐ＜０．０５），且双诱癌因素组（Ａ）灶数量明显大于单诱癌因素组（Ｂ、Ｃ）（Ｐ＜０．０５）；（２）实验第１５周，即有ＩＧＦ－Ⅱ阳性表达，在第４５周阳性率明显高于第７５周（Ｐ＜０．０５）；（３）ＨＢＶ感染组（Ａ，Ｂ）动物肝组织中ＨＢｓＡｇ阳性率高达９２．１％，首次在树肝组织中检出ＨＢｘＡｇ，且阳性率达８６．５％。提示：ＨＢＶ与ＡＦＢ１协同致树ＨＣＣ；ＨＣＣ发生早期就有ⅠＧＦ－Ⅱ异常表达。
Dynamic observation of gamma-glutamyl transpeptidase-positive hepatic foci (GGT-positive foci) and insulin-like growth factor-II (IGF-II) in hepatocellular carcinoma (HCC) lesions induced by HBV and AFB1-induced tree shrews Investigate the possible mechanism of HCC. The experimental animals were divided into 4 groups, group A: HBV positive and intake of AFB1; group B: HBV positive group; group C: intake of AFB1; group D: blank control. Histochemical and immunohistochemical detection of liver biopsy. RESULTS: (1) The number of lesions in GGT-positive lesions of precancerous lesions in all experimental groups was higher than that in the control group (P<0.05), and the number of lesions in the double-induction factor group (A) was significantly greater than that in the control group. Induction factor group (B, C) (P<0.05); (2) At the 15th week of the experiment, there was positive expression of IGF-II, and the positive rate was significantly higher at the 45th week than at the 75th week (P<0. 05); (3) The positive rate of HBsAg in the liver tissue of HBV infected group (A, B) was as high as 92.1%. HBxAg was detected in the liver tissue of the tree shrew for the first time, and the positive rate was 86.5%. Tip: HBV and AFB1 cooperate to cause tree shrew HCC; HCC early abnormal expression of IGF-II.