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目的：检测急性白血病细胞内ｐ５３基因表达情况。方法：３Ｈ－ｄＡＴＰ标记基因探针的斑点杂交。结果：急性淋巴细胞白血病（ＡＬＬ）表达ｐ５３ＲＮＡ水平与正常对照类似，急性非淋巴细胞白血病（ＡＮＬＬ）中５９．４％表达低水平ｐ５３或表达缺失，ｐ５３表达缺失在Ｍ４、Ｍ５中多见。结论：急性白血病细胞中存在ｐ５３ＲＮＡ表达异常。
Objective: To detect the expression of p53 gene in acute leukemia cells. Methods: Dot hybridization of 3H-dATP labeled gene probes. RESULTS: The expression of p53 RNA in acute lymphoblastic leukemia (ALL) was similar to that in normal controls. 59.4% of acute non-lymphocytic leukemia (ANLL) expressed low levels of p53 or lack of expression. The loss of p53 expression was more common in M4 and M5. Conclusion: There is abnormal expression of p53 RNA in acute leukemia cells.