论文部分内容阅读
本文回顾性地用原位分子杂交法检测了39例经2次以上活检才由病理确诊的鼻咽癌所有阳性和阴性切片中EBVDNA的存在。所用探针为病毒基因组中BamHI－W片段。结果表明39例鼻咽癌中36例EBV阳性，检出率为92．3％。4例癌旁上皮有不同程度的阳性，所有间质成分均为阴性。这39例鼻咽癌的43次阴性活检中11例检出了EBVDNA。对该11例本次所作的HE切片重新进行了评价，4例诊断为鼻咽癌，4例为可疑鼻咽癌。3例未见癌。根据前述结果，有理由认为这7例“可疑癌和未见癌”中EBV阳性细胞为鼻咽癌细胞。以上结果提示用原位杂交方法检测EBV为鼻咽癌诊断和鉴别诊断中一有用的工具。
In this review we retrospectively used in situ hybridization to detect the presence of EBV DNA in all 39 positive and negative nasopharyngeal carcinomas, which were pathologically diagnosed after more than two biopsies. The probe used was a BamHI-W fragment in the viral genome. The results showed that 39 cases of nasopharyngeal carcinoma EBV-positive in 36 cases, the detection rate was 92.3%. 4 cases of paracancerous epithelium have different degrees of positive, all the interstitial components are negative. EBVDNA was detected in 11 of the 43 negative biopsies from 39 cases of NPC. The 11 cases of this HE made re-evaluation of the film, 4 cases were diagnosed as nasopharyngeal carcinoma, 4 cases of suspected nasopharyngeal carcinoma. No cancer in 3 cases. Based on the above results, it is reasonable to assume that the EBV-positive cells in the seven cases of “suspicious and non-cancerous” were nasopharyngeal carcinoma cells. These results suggest that in situ hybridization detection of EBV for the diagnosis and differential diagnosis of nasopharyngeal carcinoma a useful tool.