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[Objective]To establish a simultaneous detection method for the four effective components(neochlorogenic acid,chlorogenic acid,isochlorogenic acid A and isochlorogenic acid C)in Luzhou FLOS LONICERAE.[Methods]HPLC was adopted.Chromatographic column was Kromasil C18column(250 mm×4.6 mm,5μm);mobile phase was acetonitrile-0.1%phosphoric acid solution;detection wavelength was 326 nm;column temperature was 30℃;flow rate was 1 mL/min;and injection volume was 10μL.[Results]The four components all had good linear relationships;the RSD values of precision,stability and repeatability tests were all lower than 3%;and the recovery rates were between 98.94%and 99.26%.[Conclusions]This method was accurate,sensitive and repeatable,and could be used for the quality control of Luzhou FLOS LONICERAE.
[Objective] To establish a simultaneous detection method for the four effective components (neochlorogenic acid, chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C) in Luzhou FLOS LONICERAE. [Methods] HPLC was was applied. Chromatographic column was Kromasil C18column (250 mm × 4.6 mm, 5 μm); mobile phase was acetonitrile-0.1% phosphoric acid solution; detection wavelength was 326 nm; column temperature was 30 ° C .; flow rate was 1 mL / min; and injection volume was 10 μL. [Results] The four components all had good linear relationships; the RSD values of precision, stability and repeatability tests were all lower than 3%; and the recovery rates were between 98.94% and 99.26%. [Conclusions] This method was accurate, sensitive and repeatable, and could be used for the quality control of Luzhou FLOS LONICERAE.