Detection of the Saikosaponin a and d Contents in RADIX BUPLEURI and the Establishment of Its Finger

• 作 者: Yanchun ZHENG Yahui CUI Ya'na SUN Xiaoming WANG Chunfeng YAN
• 机 构: Jingfukang Pharmaceutical Group Co.,Ltd.,Hebei Provincial Engineering Technology Research Center for
• 出 处: Medicinal Plant
• 发表日期: 2013年08期
• 关键词: decoction fingerprint acetonitrile cultivar chromato references affecting roots

[Objective]To establish the fingerprint of RADIX BUPLEURI,to investigate the differences in the chemical components in different parts of North and South RADIX BUPLEURI,and to provide references for the quality control of RADIX BUPLEURI.[Methods]The fingerprint of RADIX BUPLEURI was established by HPLC.The contents of saikosaponin a and d in RADIX BUPLEURI were detected according to the Chinese Pharmacopoeia(2010 edition).And the differences in chemical components were compared.[Results]There were significant differences in the chemical components between North and South RADIX BUPLEURI.The above-ground parts of North RADIX BUPLEURI showed significant differences with its roots in chemical components;and the content of the above-ground part directly affected the quality of decoction pieces.At the same time,there were significant differences in the chemical components among different species of South RADIX BUPLEURI;and the cultivar origin was the key factor affecting the quality of decoction pieces.[Conclusions]There were significant differences in the chemical components in different species of RADIX BUPLEURI.

[Objective] To establish the fingerprint of RADIX BUPLEURI, to investigate the differences in the chemical components in different parts of North and South RADIX BUPLEURI, and to provide references for the quality control of RADIX BUPLEURI. [Methods] The fingerprint of RADIX BUPLEURI was established by HPLC. The contents of saikosaponin a and d in RADIX BUPLEURI were detected according to the Chinese Pharmacopoeia (2010 edition). And the differences in chemical components were compared. [Results] There were significant differences in the chemical components between North and South RADIX BUPLEURI. These above-ground parts of North RADIX BUPLEURI showed significant differences with the roots in chemical components; and the content of the above-ground part directly affected the quality of decoction pieces. At the same time, there were significant differences in the the chemical components among different species of South RADIX BUPLEURI; and the cultivar origin was the key factor affecting the quality of decoc tion pieces. [Conclusions] There were significant differences in the chemical components in different species of RADIX BUPLEURI.