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目的:研究黄金牡丹合剂(HJM)对内毒素血症小鼠细胞因子的影响。方法:腹腔注射内毒素(LPS)以复制小鼠内毒素血症模型。实验分为空白(等容无菌水)、模型对照(等容无菌水)、地塞米松(DEXA,0.80mL.kg-1)和HJM高、中、低剂量(1.00、0.50、0.25mL.kg-1)组,灌胃给药,每天2次,连续3d,观察以上6组小鼠72h死亡率。采用相同给药方式,以酶联免疫吸附法(ELISA)测定复制模型后空白、模型对照、DEXA和HJM中剂量组6、12、18、24、48、72h血清肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-4、IL-6含量。结果:与模型对照组比较,HJM中剂量组死亡率显著降低(P<0.01)。HJM组TNF-α、IL-6含量显著降低(P<0.01或P<0.05),IL-4含量显著升高(P<0.05)。结论:HJM能显著降低内毒素血症小鼠死亡率,其机制可能与降低血清中TNF-α、IL-6的含量,促进IL-4释放,抑制过度炎症反应有关。
Objective: To study the effects of Golden Peony Mixture (HJM) on cytokines in endotoxemic mice. Methods: Endotoxin (LPS) was injected intraperitoneally to replicate mouse endotoxemia model. The experiments were divided into blank (equal volume sterile water), model control (equal volume sterile water), dexamethasone (DEXA, 0.80mL.kg-1) and HJM high, medium and low dose (1.00,0.50,0.25mL .kg-1) group, intragastrically administered twice a day for 3 days. The mortality of 72 h mice in the above 6 groups was observed. The same mode of administration was used to determine the level of tumor necrosis factor-α (TNF-α) in the blank, model control, DEXA and HJM middle dose groups at 6,12,18,24,48,72h after enzyme-linked immunosorbent assay (ELISA) ), Interleukin (IL) -4, IL-6 content. Results: Compared with the model control group, the mortality of HJM middle dose group was significantly lower (P <0.01). The levels of TNF-α and IL-6 in HJM group were significantly decreased (P <0.01 or P <0.05), while IL-4 levels were significantly increased (P <0.05). Conclusion: HJM can significantly reduce the mortality of mice with endotoxemia, the mechanism may be related to the decrease of TNF-α and IL-6 in serum, the release of IL-4 and the suppression of excessive inflammatory reaction.