Cloning and Expression Analysis of OsNADPH1 Gene from Rice in Drought Stress Response

• 作 者: CHEN Jing WAN Jia JIANG Hua GA
• 机 构: Rice Research Institute,Key Laboratory of Crop Genetic Resources and Improvement
• 出 处: 水稻科学（英文版）
• 发表日期: 2004年期
• 关键词: rice drought stress NADPH oxidoreductase-like gene cloning gene expression
• 基金项目: 国家自然科学基金;教育部长江学者和创新团队发展计划

An experiment was conducted to compare the mRNA expression difference in rice leaves and roots under drought stress and normal conditions using Fluorescent Differential Display (FDD) method. One positive fragment was isolated by combination of the H. A. Yellow-PAGE (contained 0.1% H. A. Yellow) separation and macroarray screening methods. Compared to Arabidopsis thaliana NADPH oxidoreductase gene, it has 96% identity. The cDNA was 1423 bp, and contained a complete open reading frame of 1048 bp encoding a protein with 345 amino acid residues. Moreover, the gene expression level was higher under drought stress than that under normal conditions. The possible role of NADPH oxidoreductase gene under drought response was also discussed.