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目的:荞麦是日本法规要求强制性标识的食品过敏原,我国需要建立出口日本食品中过敏原荞麦成分的标准方法。方法:参考现有文献,采用实时PCR方法建立食品过敏原荞麦成分的检测方法。结果:研究表明建立的检测方法具有特异性,灵敏度高,检测限为1 mg/kg。结论:本方法适用于食品中过敏原荞麦成分检测。
OBJECTIVE: Buckwheat is a food allergen that is mandatory by Japanese laws and regulations. China needs to establish a standard method for the export of allergen buckwheat ingredients in Japanese food. Methods: With reference to the existing literature, real-time PCR method was used to establish the detection method of food allergen buckwheat. Results: The study shows that the established detection method is specific and sensitive with a detection limit of 1 mg / kg. Conclusion: This method is suitable for the detection of buckwheat allergen in food.