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目的研究稀土元素镧对雄性小鼠肝脏氧化损伤作用及对肝脏组织中微量元素含量的影响。方法雄性昆明小鼠40只,随机分为4组:阴性对照组和硝酸镧低(1mg/kg)、中(5mg/kg)、高(20mg/kg)剂量组,连续灌胃染毒2周后,测定肝脏组织中还原型谷胱甘肽(GSH)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。另取肝脏组织消化处理后,火焰原子吸收光谱法测定组织中铜(Cu)、锌(Zn)、锰(Mn)、铁(Fe)4种金属离子含量。结果硝酸镧染毒可明显降低雄性小鼠肝脏组织中GSH含量和SOD活性,提高MDA含量;小鼠肝脏组织微量元素测定结果显示:各剂量硝酸镧染毒组Cu、Zn含量明显降低,Fe含量有增加趋势,Mn含量与对照组比较差异无统计学意义。结论硝酸镧可造成雄性小鼠肝脏脂质过氧化损伤,这可能与镧干扰了Cu、Zn、Fe在肝脏内的分布和蓄积,进而抑制抗氧化酶活性,增强自由基活性有一定关系。
Objective To study the effect of rare earth element lanthanum on the oxidative damage of liver and the content of trace elements in liver of male mice. Methods Forty male Kunming mice were randomly divided into 4 groups: negative control group and lanthanum nitrate low dose (1mg / kg), medium dose (5mg / kg), high dose (20mg / kg) Afterwards, the contents of reduced glutathione (GSH), malondialdehyde (MDA) and superoxide dismutase (SOD) in the liver tissue were measured. In addition, the contents of Cu, Zn, Mn and Fe in the tissue were determined by flame atomic absorption spectrometry after digestion of liver tissues. Results The lanthanum nitrate treatment significantly reduced GSH content and SOD activity in liver tissue of male mice and increased the content of MDA. The results of trace elements assay in liver showed that the content of Cu and Zn in the group treated with lanthanum nitrate decreased significantly, while the content of Fe There is an increasing trend, Mn content and the control group was no significant difference. Conclusion Lanthanum nitrate can cause lipid peroxidation injury in male mice liver, which may interfere with the distribution and accumulation of Cu, Zn and Fe in the liver with lanthanum, thereby inhibiting the activity of antioxidant enzymes and enhancing the activity of free radicals.