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目的:探讨长链非编码RNA LINC00668在胃癌中的表达及作用。方法:在深圳市人民医院收集2018年6月至2019年5月115例行胃癌根治术患者的癌及癌旁组织，采用实时定量反转录聚合酶链反应(RT-qPCR)检测LINC00668的表达，并分析其与患者临床病理特征的关系。通过细胞计数试剂盒(CCK-8)和克隆形成实验检测LINC00668对胃癌细胞系BGC-823的影响，进一步裸鼠皮下移植瘤模型验证LINC00668对胃癌生长速度的影响。结果:比较采用独立样本n t检验。两组率的比较采用n χ2检验。结果 胃癌组织中LINC00668的表达(9.62±0.35)显著高于癌旁组织(5.50±0.24，n t＝9.800，n P0.05；n χ2＝0.071，n P>0.05；n χ2＝2.580，n P>0.05)。干扰LINC00668后，阴性对照组和shRNA干扰组克隆形成数目分别为(309.00±12.01)个和(125.00±8.95)个，shRNA干扰组的细胞增殖、克隆形成和裸鼠皮下移植瘤生长速度均显著低于阴性对照组(n t＝7.723，n P<0.05；n t＝4.719，n P<0.05；n t＝3.790，n P<0.05)。n 结论:长链非编码RNA LINC00668在胃癌中可能促进癌细胞的生长。“,”Objective:To investigate the expression and role of long non-coding RNA (lncRNA) LINC00668 in gastric cancer.Methods:From June 2018 to May 2019, the cancer tissues and matched normal gastric tissue samples of 115 gastric cancer patients in Shenzhen People′s Hospital were collected. The expression level of LINC00668 in the tissue samples was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). The correlation between the expression and clinicopathological factors was analyzed. The proliferation ability of gastric cancer cell line BGC-823 transfected with short hairpin RNA (shRNA) was detected by cell counting kit-8 (CCK-8) assay and plate cloning. Nude mice were transplanted with shRNA and negative shRNA, respectively. Tumor volume was measured. SPSS 16.0 was used for statistical analysis in this study.Results:The expression of LINC00668 in gastric cancer tissues (9.62±0.35) was significantly higher than that in normal gastric tissues (5.50±0.24, n t=9.800, n P<0.05). The expression level of LINC00668 in gastric cancer tissue was correlated with tumor diameter, invasion depth and TNM stage (n χ2=10.671, n P<0.001;n χ2=4.614, n P<0.032;n χ2=7.398, n P<0.007). BGC-823 cell line was successfully transfected with shRNA. The number of clones in the negative control group and shRNA interference group was (309.00±12.01) and (125.00±8.95) respectively. LncRNA LINC00668 could significantly reduce cell proliferation (n t=7.723, n P<0.05), clonal formation (n t=4.719, n P<0.05) and tumor growth (n t=3.790, n P<0.05).n Conclusion:LncRNA LINC00668 could promote the growth of gastric cancer, and could be a potential therapeutic target gene.