Simultaneous Determination of Four Components in Yinzhihuang Granules by HPLC

• 作 者: Chunhong LI Bing HE Jinping ZHANG Ji TIAN
• 机 构: Research Center for Drug and Functional Food of Luzhou Medical College,Department of Pathogenic Biol
• 出 处: Medicinal Plant
• 发表日期: 2013年03期
• 关键词: granules methanol simultaneous endowed dissolved shares batch extracts gallbladd

[Objective] To establish an HPLC method for the simultaneous determination of the contents of Baicalin, Wogonoside, Baicalein, Wogonin in Yinzhihuang granules. [Methods] The separation was performed by HPLC method using Dikma Diamonsil C18 column (250 mm×4.6 mm, 5 μm), mobile phase of methanol-0.1% phosphoric acid with gradient elution and detection wavelength at 278 nm. [Results] The linear ranges of Baicalin, Wogonoside, Baicalein, Wogonin in Yinzhihuang granules were determined as 0.418-4.18 μg/mL(r=0.999 8), 0.2-2 μg/mL(r=0.999 9), 0.233-2.33 μg/mL(r=0.999 8), 0.215-2.15 μg/mL(r=0.999 8), repsectively.The average recoveries were of Baicalin, Wogonoside, Baicalein, Wogonin were 98.40%(RSD=0.32%), 98.72%(RSD=1.37%), 98.70%(RSD=1.55%), 98.78%(RSD=1.24%). [Conclusion] This method is simple, rapid, accurate and of good repeatability and high recovery, and it can be used for quantitative analysis of Yinzhihuang granules.