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本文介绍血浆总胆固醇浓度的气液色谱(GLC)测定法。血浆用量为50微升,皂化后用己烷提取。除去提取液中的己烷,加入十八甲基炔诺酮(内标液),混均。进样1微升。血浆总胆固醇峰高与内标物峰高的比值和标准液胆固醇浓度呈线性关系。变异系数3.1％。相对误差3.0％和比色法比较,结果一致。GLC法操作简便、准确、精密和专一,血浆用量尚可进一步减少,适合临床以及实验研究使用。
This article describes the gas chromatography (GLC) assay for plasma total cholesterol concentration. The amount of plasma was 50 μl, extracted with hexane after saponification. Remove the hexane extract, octadecline norethindrone (internal standard solution), mixed. Inject 1 μl. Plasma total cholesterol peak height and internal standard peak height ratio and the standard solution of cholesterol concentration showed a linear relationship. The coefficient of variation is 3.1%. The relative error of 3.0% and colorimetric method, the results are consistent. GLC method is simple, accurate, precise and specific, and the plasma dosage can be further reduced, which is suitable for clinical and experimental research.