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用计算机辅助医学显微图像分析仪定量检测106例初发白血病患者骨髓细胞的核仁形成区相关嗜银蛋白(Ag-NORs),并与同标本中的抗人增殖细胞Ki-67阳性率、S+G2M细胞比例,DNA倍体进行比较,发现Ag-NORs与Ki-67阳性率。S+G2M细胞比例有相关关系,与DNA倍体无显著相关关系。Ag-NORs在白血病组高于正常对照组:急性白血病组高于慢性白血病;急性淋巴细胞白血病高于急性非淋巴细胞自血病;急性早幼粒细胞白血病最低。在慢性白血病中,慢性粒细胞白血病显著高于慢性淋巴细胞白血病;早幼粒细胞白血病缓解期显著低于治疗前,复发时又显著增高。结果显示:Ag-NORs可以和Ki-67免疫标记、细胞流式仪一样,作为白血病诊断,病情监测及预后判断的一项参考指标。
Using computer assisted medical microscopic image analyzer quantitative detection of 106 cases of primary leukemia in patients with bone marrow cells of nucleolar organizer area associated with Ag-NORs, and with the same specimens of anti-human proliferating cells Ki-67 positive rate, S + G2M cell ratio, DNA ploidy were compared and found that Ag-NORs and Ki-67-positive rate. There was a correlation between the proportion of S + G2M cells and no significant correlation with DNA ploidy. Ag-NORs in leukemia group than the normal control group: acute leukemia group than chronic leukemia; acute lymphoblastic leukemia than acute non-lymphocytic leukemia; acute promyelocytic leukemia the lowest. In chronic leukemia, chronic myeloid leukemia was significantly higher than chronic lymphocytic leukemia; promyelocytic leukemia remission was significantly lower than before treatment, relapse was significantly higher. The results showed that Ag-NORs can be used as a reference index for the diagnosis, prognosis and prognosis of leukemia in the same way as Ki-67 immunofluorescence and cell flow cytometer.