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以垦丰14、垦丰15、垦丰19和黑农48为亲本,构建了含160个株系的四向重组自交系群体(four-way recombinant inbred lines,FW-RIL)为试验材料,通过构建分子遗传连锁图谱,对大豆四向重组自交系群体生育期各阶段进行QTL定位,旨在为大豆生育期性状的分子辅助育种提供理论基础和技术支撑,在分子水平探索与大豆生育期相关基因的遗传机理。通过区间作图法等分析方法,应用275个SSR分子标记对2013、2014与2015年在克山和哈尔滨3年3个环境下的生育期各阶段进行QTL定位,结果表明:大豆四向杂交群体的各生育期阶段易受到环境条件的影响,生育期相关性状在Fw-RIL群体中存在真实的遗传变异。76个与生育期各阶段相关的QTL分布在18个连锁群上,分别是A1、A2、B1、B2、C1、C2、D1a、D1b、D2、F、G、H、J、K、L、M、N和O连锁群;17个与生育期各阶段相关的QTL能够在2个以上环境中被重复定位到,分别为qST-B2-1、qST-C1-1、qST-C2-1、qST-C2-2、qST-D1b-1、qST-D1b-2、qST-G-1、qST-H-1、qST-K-1、qST-K-2、qST-L-1、qST-L-2、qST-M-1、qST-M-2、qST-M-3、qST-N-1、qST-O-1。其中,新发现10个与生育期相关的QTLs,qST-C2-2,qST-D1b-1,qST-k1,qST-M2和qST-N1是高效QTL。
Four-way recombinant inbred lines (FW-RIL) containing 160 lines were constructed with Kenfeng 14, Kenfeng 15, Kenfeng 19 and Heinong 48 as experimental materials, Through the construction of molecular genetic linkage map, QTL mapping of soybean four-way recombinant inbred lines at all stages of development was carried out to provide theoretical basis and technical support for the molecular-assisted breeding of soybean growth traits. At the molecular level to explore and soybean growth stage Related gene genetic mechanism. Using interval mapping method and other analysis methods, 275 SSR markers were used to map the QTLs at different growth stages in three years in Keshan and Harbin in 2013, 2014 and 2015 respectively. The results showed that: Were susceptible to environmental conditions at each growth stage, and there was a true genetic variation in the Fw-RIL population for the traits related to growth. 76 QTLs associated with each stage of growth period were distributed in 18 linkage groups, which were A1, A2, B1, B2, C1, C2, D1a, D1b, D2, F, G, H, J, K, M, N and O linkage groups. Seventeen QTLs related to each stage of reproductive development could be repeatedly mapped in more than two environments, namely QST-B2-1, qST-C1-1, qST-C2-1, qST-C2-2, qST-D1b-1, qST-D1b-2, qST- G- 1, qST- H- 1, qST- K- 1, qST- K- 2, qST- L- 1, qST- L-2, qST-M-1, qST-M-2, qST-M-3, qST-N-1, qST-O-1. Among them, ten new QTLs related to growth period were found, qST-C2-2, qST-D1b-1, qST-k1, qST-M2 and qST-