论文部分内容阅读
目的:探讨不同氟暴露水平地区汉族人群卵泡刺激素受体(FSHR)基因rs1394205多态性与血清钙、镁含量的关系。方法:采用整群抽样方法分别在氟病区(305例)、改水区(250例)和对照区(465例)选择居住5 a以上18~55岁者为观察人群,采用Real-Time PCR方法检测FSHR基因rs1394205多态性;采用氟离子选择电极法检测人群尿氟含量,采用原子吸收分光光度法检测血清钙、镁含量。结果:不同氟暴露组男性人群之间血清钙、镁含量差异有统计学意义(F=8.713、6.553,P均<0.05),不同氟暴露组女性人群之间血清钙、镁含量差异有统计学意义(F=6.760、6.879,P均=0.001)。男性人群中,携带AG基因型者改水组血清钙含量低于对照组和病区组,携带GG基因型者改水组血清镁含量低于对照组和病区组(P均<0.05)。女性人群中,携带AA、AG基因型者改水组血清镁含量低于对照组和病区组(P均<0.05)。结论:氟暴露和FSHR基因rs1394205多态性的相互作用可能影响人群血清钙、镁含量。
Objective: To investigate the relationship between the rs1394205 polymorphism of follicle stimulating hormone receptor (FSHR) gene and serum levels of calcium and magnesium in Han population of different fluoride exposure levels. Methods: A total of 305 patients in the ward, 250 in the water area and 465 in the control group were enrolled. Methods The rs1394205 polymorphism of FSHR gene was detected by enzyme-linked immunosorbent assay (ELISA). Urine fluoride content was measured by fluoride ion selective electrode method. The contents of calcium and magnesium in serum were detected by atomic absorption spectrophotometry. Results: There were significant differences in serum calcium and magnesium levels among different fluoride exposure groups (F = 8.713, 6.553, P <0.05). The differences of serum calcium and magnesium levels among different fluoride exposure groups were statistically significant Significance (F = 6.760, 6.879, P = 0.001). In the male population, the serum calcium levels in the water-modified group with AG genotype were lower than those in the control group and the ward group. The levels of serum magnesium in the water-modified group with GG genotype were lower than those in the control group and the ward group (all P <0.05). In female population, serum magnesium levels in AA and AG genotypes were lower than those in control group and ward group (P <0.05). Conclusion: The interaction between fluoride exposure and rs1394205 polymorphism of FSHR gene may affect serum calcium and magnesium levels in the population.