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现认为自由基反应与缺血性心肌损害关系密切。为探讨丹参、红藤、当归、黄芪等中药防治缺血性心脏病有效的机制,本文采用了黄嘌呤—黄嘌呤氧化酶—鲁米诺化学发光体系,用化学发光测量仪测定发光强度,以检测药物的抗自由基效果。实验结果发现上述四种药物均有抗自由基的作用,其LC50(药物抑制发光强度50%的浓度)分别为丹参酮Ⅱ_A磺酸钠1.90微克/毫升、红藤多糖0.75微克/毫升、当归水提取物62.5微克/毫升、黄芪水提取物130.0微克/毫升,提示抗自由基作用是上述四种药物防治疾病机理之一。本文报告了用化学发光检测药物抗自由基作用的具体方法,此方法简便、灵敏、价廉、可靠,值得推广。
It is thought that free radical reaction is closely related to ischemic myocardial damage. To investigate the effective mechanisms of Chinese traditional medicines such as Salvia miltiorrhiza, Hongteng, Angelica sinensis and Radix Astragali in the prevention and treatment of ischemic heart disease, the xanthine-xanthine oxidase-luminol chemiluminescence system was used in this study. The chemiluminescence intensity was measured with a chemiluminescence measuring instrument. Test the drug’s anti-free radical effects. The experimental results showed that the above four drugs all had anti-free radical effects. The LC50 (concentration of 50% inhibition of luminescent intensity of the drug) was 1.90 μg/ml sodium tanshinone II_A sulfonate, 0.75 μg/ml red rattan polysaccharide, and water extract of Angelica sinensis. 62.5 μg/ml of substance and 130.0 μg/ml of Astragalus membranaceus extract suggested that the anti-free radical effect is one of the mechanisms of the above four drugs to prevent diseases. This article reports a specific method for detecting the anti-radical effect of drugs using chemiluminescence. This method is simple, sensitive, inexpensive and reliable and worthy of promotion.