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用离心薄层层析法从灌胃大黄素甲醚的大鼠和小鼠尿中分离了六种代谢产物。经质谱、高效液相色谱及光谱测定并与部分标准品对照,确定其结构分别为大黄素、大黄酚,3-羟甲基-1,6,8-三羟基葸醌,3-甲酰基-1,6,8-三羟基蒽醌,3-羧基-1,6,8-三羟基蒽醌,3-羧基-6-甲氧基-1,8-二羟基葸蒽。大鼠和小鼠肝匀浆9000×g上清液的体外代谢实验也获得了与上述前五种相同的代谢产物。实验表明大黄素甲醚在C-6位可脱去甲基或甲氧基,在C-3位的甲基可相继氧化为羟甲基、甲酰基和羧基等极性更强化合物。
Six metabolites were isolated from rat rheumatoid-treated rats and urine by centrifugal thin-layer chromatography. After mass spectrometry, high performance liquid chromatography and spectrometry, and compared with some standards, the structures were determined to be emodin, chrysophanol, 3-hydroxymethyl-1,6,8-trihydroxyanthraquinone, 3-formyl- 1,6,8-trihydroxy oxime, 3-carboxy-1,6,8-trihydroxy oxime, 3-carboxy-6-methoxy-1,8-dihydroxy oxime. In vitro metabolic experiments of rat and mouse liver homogenates of 9000 xg supernatant also yielded the same metabolites as the first five above. Experiments show that emodin can be demethylated or methoxy at the C-6 position, and the C-3 methyl group can be sequentially oxidized to more polar compounds such as hydroxymethyl, formyl, and carboxyl groups.