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细胞外基质的理化性能影响着细胞的命运并调节组织形成。为探索最佳的软骨细胞体外三维生长环境,本研究通过改变藻酸盐浓度测定了海藻酸盐小球的力学特性及其对软骨细胞增殖能力的影响。凝胶的压缩机械性能通过INSTRON3365测定,结果发现浓度越高其压缩模量越大,表明凝胶对抗应变的能力越强。同时选取4w龄乳兔关节软骨细胞分别包裹在浓度为1%、2%和3%(w/v)海藻酸盐小球中连续培养4周,结果发现所有3组软骨细胞都表现出有限的增殖并形成类似于软骨组织的细胞簇,其中较低浓度组(1%和2%)增殖更明显。这些结果表明软骨细胞在海藻酸盐凝胶小球中连续培养有少量增殖,且较低浓度的藻酸盐更有利于软骨细胞的增殖,说明藻酸盐凝胶的力学特性影响了软骨细胞的增殖能力。
The physicochemical properties of the extracellular matrix influence the fate of cells and regulate tissue formation. In order to explore the best three-dimensional growth environment of chondrocytes in vitro, we studied the mechanical properties of alginate beads and their effects on the proliferation of chondrocytes by changing the concentration of alginate. Compression mechanical properties of the gel were determined by INSTRON 3365 and found that the higher the concentration the greater the compressive modulus, indicating that the gel is more resistant to strain. At the same time, 4-week-old rabbit articular chondrocytes were selected and incubated in alginate pellets of 1%, 2% and 3% (w / v) concentration respectively for 4 weeks. All three groups of cartilage cells showed limited Proliferated and formed clusters of cells similar to cartilage tissue in which proliferation was more pronounced in lower concentrations (1% and 2%). These results indicate that chondrocytes continue to proliferate slightly in alginate gel beads, and lower concentrations of alginate are more beneficial for chondrocyte proliferation, indicating that the mechanical properties of alginate gels affect chondrocyte Proliferative ability.