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目的：研究人肺癌、成人肺及胚肺组织基因表达谱差异，观察肺癌发生中多个基因的作用。方法：用鳞状细胞肺癌、胎儿胚肺、胸外伤部分肺切除肺组织，提取总ＲＮＡ 后逆转录标记ｃＤＮＡ 探针，与代表５８８ 个基因的ＡｔｌａｓＴＭ ｃＤＮＡ 阵列杂交，灰度扫描杂交信号强弱及统计分析差异。结果：肺癌组织中ｃｍｙｃ、ｃｆｏｓ 相关基因等多个较正常肺组织表达增加的基因，其中有些基因表达变化与胚胎肺组织相似。结论：多个基因的表达变化在肺癌发生中起作用，且与胚肺基因表达谱相似，而表现出恶性肿瘤发生中的“胚胎化”生物学特性。
Objective: To study the differences of gene expression profiles between human lung cancer, adult lung and embryonic lung tissue, and to observe the role of multiple genes in the development of lung cancer. METHODS: Lung tissues were resected with squamous cell lung cancer, fetal embryo lungs, and thoracic injuries. Total RNA was extracted and the cDNA probes were reverse transcribed. Hybridized with At lasTM cDNA arrays representing 588 genes. Strong gray-scanning hybridization signals. Weak and statistical analysis differences. RESULTS: The expression of c-myc and c-fos related genes in lung cancer tissues was higher than that in normal lung tissues, and some of them were similar to embryonic lung tissues. Conclusion: The expression changes of multiple genes play a role in the development of lung cancer, and similar to the gene expression profile of embryo lungs. It shows the biological characteristics of “embryogenesis” in the development of malignant tumors.