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[Objective] To optimize the extraction technology of tannins from Ziziphus jujuba roots, and to establish stable determination method of tannins content in Z. jujuba roots. [Method] Content of total tannins was detected by casein method. With tannins content as the evaluation index, the extraction time, solid-liquid ratio, extraction times, and acetone concentration were taken as the investigation factors. Extraction technology conditions were optimized by orthogonal design. [Result] Results showed that extraction times was the major influencing factor. The optimal extraction condition was as follows: 70% acetone concentration, 1∶20(W/V, g/mL)solid-liquid ratio, 30 min extraction time and 3 extraction times. Under this condition, the tannins content in Z. jujuba roots reached 6.82%. [Conclusion] The optimized extraction technology was reasonable, simple and feasible.
[Objective] To optimize the extraction technology of tannins from Ziziphus jujuba roots, and to establish stable determination method of tannins content in Z. jujuba roots. [Method] Content of total tannins was detected by casein method. With tannins content as the evaluation index , the extraction time, solid-liquid ratio, extracted times, and acetone concentration were optimized by orthogonal design. [Result] Results showed that extraction times was the major influencing factor. The optimal extraction condition The results are as follows: 70% acetone concentration, 1:20 (W / V, g / mL) solid-liquid ratio, 30 min extraction time and 3 extraction times. Under this condition, the tannins content in Z. jujuba roots reached 6.82% . [Conclusion] The optimized extraction technology was reasonable, simple and feasible.