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目的观察三氯乙烯(TCE)对人周围血T淋巴细胞活化的生物学效应。方法纯化人周围血T淋巴细胞,高、中、低剂量组分别给予1 000、500、250μmol/L TCE刺激;另设未加TCE的对照组作为阴性对照组,加入佛波酯与钙离子载体的对照组作为阳性对照组。采用流式细胞术检测T淋巴细胞活化表面标志物CD69阳性细胞占所有细胞的百分率(M1)。酶联免疫吸附实验检测白细胞介素-2(IL-2)的蛋白表达量,实时定量聚合酶链反应技术检测IL-2 mRNA表达量。结果与阴性对照组比较,高、中剂量组M1均增加[(37.7±1.4)%、(20.7±3.0)%vs(6.5±1.6)%,P<0.01];高、中剂量组IL-2蛋白表达量均增加[(635.5±86.5)、(286.6±24.6)vs(40.0±9.5)ng/L,P<0.01];高剂量组IL-2 mRNA表达量增加[(497.7±67.6)vs(140.7±8.0)ng/L,P<0.01〗。结论 TCE能够促进人周围血T淋巴细胞的活化,这可能是其导致药疹样皮炎发生的重要机制。
Objective To observe the biological effects of trichlorethylene (TCE) on the activation of peripheral blood T lymphocytes in human. Methods Peripheral blood T lymphocytes were purified from human peripheral blood mononuclear cells (T-lymphocytes). TCE-stimulated cells were treated with 1, 500, 500 and 250μmol / L TCE respectively. The control group without TCE was used as a negative control group and phorbol ester and calcium ionophore Of the control group as a positive control group. Flow cytometry was used to detect the percentage of all cells (T 1), which is the activation of T lymphocyte activation surface marker CD69. The expression of interleukin-2 (IL-2) protein was detected by enzyme-linked immunosorbent assay, and the expression of IL-2 mRNA was detected by real-time quantitative polymerase chain reaction. Results Compared with the negative control group, M1 in high and middle dose groups increased (37.7 ± 1.4)%, (20.7 ± 3.0)% vs (6.5 ± 1.6)%, P <0.01; The expression of IL-2 mRNA in high-dose group was significantly higher than that in high-dose group ([(635.5 ± 86.5) vs (286.6 ± 24.6 vs 40.0 ± 9.5) ng / L, P <0.01] 140.7 ± 8.0) ng / L, P <0.01. Conclusion TCE can promote the activation of peripheral blood T lymphocytes in peripheral blood, which may be one of the important mechanisms that lead to drug-induced dermatitis.