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本研究利用两个具有粒型差异的籼稻材料14931和R1961进行杂交、自交获得F2代分离群体。利用在亲本间有多态性的102对SSR标记进行基因型分析,同时对粒长、粒宽、长宽比和粒重4种性状进行了表型鉴定,并采用完备区间作图法(ICIM)对上述性状进行QTL定位。结果表明:上述粒型性状在F2群体均呈正态连续分布,表现为由多基因控制的数量性状,共检测到12个与粒型性状相关的QTLs,分布于第1、第2、第5、第8、第10和第11染色体上。除qgl2-1和qgl2-2之外,其余有10个增效等位基因来自于小粒亲本材料R1946。粒长、粒宽和粒重均分别检测到4个QTLs,但籽粒长宽比未能检测到QTL。其中,qgl1、qgw5-1和qkgw5-1对表型变异的贡献率分别为14.55%、26.28%和15.25%,为主效QTL,并且检测到控制粒长的qgl1可能是新的QTL位点。研究结果可为进一步解析和克隆新的粒型QTLs奠定基础。
In this study, two grain-differentiated indica rice varieties 14931 and R1961 were used for crossbreeding, and F2 segregation population was obtained by selfing. 102 pairs of SSR markers with polymorphism among parents were used for genotypic analysis, and the phenotypes of four traits including grain length, grain width, aspect ratio and grain weight were identified. The complete interval mapping (ICIM QTL mapping of these traits. The results showed that all of the above-mentioned grain-type traits were normally and continuously distributed in F2 population, showing quantitative traits controlled by multiple genes. A total of 12 QTLs associated with grain traits were detected in the first, second, fifth , 8th, 10th and 11th chromosomes. With the exception of qgl2-1 and qgl2-2, the remaining 10 synergistic alleles were from the small parent material R1946. Four QTLs were detected for grain length, grain width and grain weight, respectively, but the QTL was not detected by grain aspect ratio. Among them, the contribution rates of qgl1, qgw5-1 and qkgw5-1 to phenotypic variation were 14.55%, 26.28% and 15.25% respectively, which were the main effect QTLs, and qgl1 with controlled grain length could be a new QTL locus. The results provide the basis for further analysis and cloning of new grain-type QTLs.