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利用聚丙烯酰胺凝胶电泳技术检测了浙江金华北山南坡（东经１１９°３８′，北纬２９°１３′）野生大豆（Ｇｌｙｃｉｎｅｓｏｊａ Ｓｉｅｂ． ＆ Ｚｕｃｃ．）种群内１４ 个样本６种同工酶水平上的生化遗传结构及其变异，结果表明野生大豆种群内存在大量的遗传变异。苹果酸脱氢酶（ＭＤＨ）、过氧化物酶（ＰＥＲ）、腺苷三磷酸酶（ＡＴＰａｓｅ）、淀粉酶（ＡＭＹ）表现出多态性，其中以ＡＴＰａｓｅ在种群中的变化最大，其多样性指数（ＰＩ）为０．１５８２。酯酶（ＥＳＴ）和异柠檬酸脱氢酶（ＩＤＨ）呈现单态性。野生大豆种群的平均群体杂合度为０．３１４１，样本间平均遗传距离为０．１５１２。利用聚类分析和典范分析等研究手段，结果表明各样本的生化遗传结构与环境之间不存在显著的相关性；探讨了野生大豆种群内高度遗传多态现象的成因——突变，及其维持机制——随机漂变；就野生大豆遗传资源保存的取样策略进行了讨论并提出建议
Polyacrylamide gel electrophoresis was used to detect the levels of 6 isozymes in 14 samples in the wild soybean (Glycinesojasb. & Zucc.) On the southern slopes of Jinbei, Jinhua, Zhejiang (119 ° 38 ’E and 29 ° 13’ N) The results showed that there were a large number of genetic variations in the wild soybean population. MDH, PER, ATPase and AMY showed polymorphism, of which ATPase had the largest change in the population, and its diversity The index (PI) is 0.1582. Esterase (EST) and isocitrate dehydrogenase (IDH) showed monomorphism. The average population heterozygosity of wild soybean population was 0.3141, and the average genetic distance among samples was 0.1512. Using cluster analysis and canonical analysis, the results showed that there was no significant correlation between the biochemical genetic structure of each sample and the environment. The causes and mutations of the high genetic polymorphism in the wild soybean population and its maintenance Mechanisms - random drift; sampling strategies for conserving wild soybean genetic resources were discussed and recommendations made