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作为植物信号分子的茉莉酸(JA)参与植物的发育与代谢过程,丙二烯环化酶(AOC)是JA合成途径的关键酶。在本研究中,利用同源克隆结合RACE技术从金鱼草中克隆AOC基因,命名为Am AOC。Am AOC基因开放阅读框长762 bp,由254个氨基酸残基组成,该基因包含一个Alleneoxcyc结构域,有两个内含子和三个外显子。同源比对分析结果表明Am AOC与其他植物的AOC有较高的同源性。Am AOC基因在不同组织中的表达分析发现金鱼草花中的Am AOC相对表达量较高,不同花期金鱼草RT-PCR表明,花蕾期的Am AOC相对表达量最高,盛花期的不同花器官荧光定量PCR分析表明,金鱼草花筒和花萼中Am AOC相对表达量较高。
As a plant signaling molecule, jasmonic acid (JA) is involved in plant development and metabolism. Acycline cyclase (AOC) is a key enzyme in JA synthesis pathway. In this study, AOC gene was cloned from snapdragon by homologous cloning and RACE technology and named as Am AOC. The open reading frame of the Am AOC gene is 762 bp in length and consists of 254 amino acid residues. The gene contains an Alleneoxcyc domain with two introns and three exons. Homologous alignment analysis showed that Am AOC shared higher homology with AOC of other plants. The Am AOC gene expression in snapdragon flower was higher than Am AOC gene in different tissues. The RT-PCR of snapdragon flowers at different flowering stages showed that Am AOC relative expression was the highest in flower bud stage. Fluorescence quantitative PCR analysis showed that Am AOC relative expression level in the antirrhinum and calyx of snapdragon was higher.