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目的探讨胃肠道间质瘤(gastrointestinal stormal tumors,GISTs)中磷酸化细胞外信号调节激酶(phosphorylated extracellular signal regulated kinase,p-ERK1/2)、细胞周期蛋白D1(CyclinD1)和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)蛋白的表达及其与临床病理因素的关系。方法采用免疫组织化学方法检测50例GISTs患者及肿瘤旁正常组织(平滑肌)中p-ERK1/2、CyclinD1和PCNA的表达水平,并进行比较分析。结果 GISTs组织中p-ERK1/2、CyclinD1和PCNA蛋白阳性表达率(82.0%、62.0%、66.0%)明显高于肿瘤旁正常组织(24.0%、30.0%、38.0%),差异有统计学意义(P<0.05)。随着肿瘤直径增大,p-ERK1/2、CyclinD1和PCNA蛋白阳性表达率逐渐增高,差异有统计学意义(P<0.05)。不同性别的GISTs患者CyclinD1蛋白的表达差异有统计学意义(P<0.05)。不同年龄、民族及发生部位的GISTs患者p-ERK1/2、CyclinD1和PCNA蛋白的表达差异无统计学意义(P>0.05)。相关性分析显示,p-ERK1/2(r=0.291,P<0.05)和PCNA(r=0.378,P<0.05)蛋白表达与肿瘤分级呈正相关。结论 ERK1/2激活、CyclinD1和PCNA高表达对肿瘤细胞的增殖、浸润和转移有一定的促进作用。
Objective To investigate the expression of phosphorylated extracellular signal regulated kinase (p-ERK1 / 2), cyclin D1 and proliferating cell nuclear antigen in gastrointestinal stomal tumors (GISTs) cell nuclear antigen (PCNA) protein and its relationship with clinicopathological factors. Methods The expressions of p-ERK1 / 2, CyclinD1 and PCNA in 50 cases of GISTs and adjacent normal tissues (smooth muscle) were detected by immunohistochemistry and compared with each other. Results The positive rate of p-ERK1 / 2, CyclinD1 and PCNA in GISTs was significantly higher than that in normal tissues (24.0%, 30.0%, 38.0%), the difference was statistically significant (P <0.05). The positive expression rate of p-ERK1 / 2, CyclinD1 and PCNA gradually increased with the increase of tumor diameter, the difference was statistically significant (P <0.05). The expression of CyclinD1 in GISTs with different genders was significantly different (P <0.05). There was no significant difference in the expressions of p-ERK1 / 2, CyclinD1 and PCNA in GISTs patients of different ages, nationalities and regions (P> 0.05). Correlation analysis showed that the protein expressions of p-ERK1 / 2 (r = 0.291, P <0.05) and PCNA (r = 0.378, P <0.05) were positively correlated with tumor grade. Conclusion ERK1 / 2 activation, high expression of CyclinD1 and PCNA may promote the proliferation, invasion and metastasis of tumor cells.