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目的探讨不同方法建立兔室壁瘤模型特点及其应用价值。方法新西兰白兔80只随机法分为4组,每组20只。根据不同开胸方式和结扎不同冠脉建立兔室壁瘤动物模型。胸骨开胸左前降支组采用剪开正中胸骨开胸,结扎兔左前降支动脉中段;胸骨开胸左前降支及左旋支组采用剪开正中胸骨开胸,结扎兔左前降支和左旋支动脉中段;肋间隙开胸左前降支组采用左侧胸腔第3~4肋间隙开胸,结扎兔左前降支动脉中段;肋间隙开胸左前降支及左旋支组采用左侧胸腔第3~4肋间隙开胸,结扎兔左前降支及左旋支动脉中段。饲养4w后,应用实时三维超声心动图进行模型评价,比较不同开胸方式和结扎不同冠脉建立兔室壁瘤模型的成功率、存活率及室壁瘤形成特点。结果 (1)胸骨开胸左前降支组、肋间隙开胸左前降支组动物存活率(90%、95%)明显高于胸骨开胸左前降支及左旋支组与肋间隙开胸左前降支及左旋支组(65%、70%)(P<0.05)。(2)胸骨开胸左前降支及左旋支组、肋间隙开胸左前降支及左旋支组室壁瘤形成率(84%、86%)明显高于胸骨开胸左前降支组与肋间隙开胸左前降支组(50%、53%)(P<0.05)。(3)胸骨开胸左前降支及左旋支组、肋间隙开胸左前降支及左旋支组左室舒张末期容积(LVEDV)、左室收缩末期容积(LVESV)、室壁瘤容积(LVAV)较胸骨开胸左前降支组与肋间隙开胸左前降支组明显增加(P<0.05),LVEF明显下降(P<0.05)。结论结扎不同冠状动脉对建立室壁瘤模型动物存活率和造模成功率有直接的影响,结扎左前降支和左旋支建立室壁瘤模型的模型成功率较高,可形成容积较为固定的室壁瘤。
Objective To explore the characteristics and application of different methods to establish rabbit ventricular aneurysm model. Methods Eighty New Zealand white rabbits were randomly divided into 4 groups (20 in each group). According to different thoracotomy methods and ligation of different coronary artery wall rabbit tumor model. Thoracic and thoracotomy in the left anterior descending artery group was performed with open central sternum thoracotomy, and the middle of the left anterior descending artery was ligated. Thoracic thoracotomy in the left anterior descending artery and the left circumflex artery was performed by cutting open the median thoracotomy and ligating the left anterior descending artery and the left circumflex artery In the intercostal space, the left anterior descending branch of the intercostal space was opened with the 3rd to 4th intercostal space in the left thoracic cavity and the middle of the left anterior descending artery was ligated in the left intercostal space. Intercostal space thoracotomy, left anterior descending artery and left circumflex artery ligation. After feeding for 4 weeks, real-time three-dimensional echocardiography was used to evaluate the model. The success rate, survival rate and the formation of aneurysm were compared between different thoracotomy methods and ligation of different coronary arteries. Results (1) Thoracic and thoracic left anterior descending coronary artery group. The survival rate of the left anterior descending coronary artery in the intercostal space (90%, 95%) was significantly higher than that in the left anterior descending coronary artery and left circumflex artery thoracotomy. Branch and L-branch group (65%, 70%) (P <0.05). (2) Thoracic and thoracic left anterior descending artery and left circumflex artery group, the rate of the formation of left and right anterior descending artery and left circumflex artery in intercostal space was significantly higher than that in left anterior descending coronary artery and intercostal space Thoracotomy in the left anterior descending branch group (50%, 53%) (P <0.05). (3) LVEDV, LVESV, LVAV of left anterior descending branch and left circumflex artery in intervertebral space were significantly higher than those in control group (P <0.05), but LVEF was significantly lower than that in the left anterior descending branch of the sternum and the left anterior descending branch of the intercostal space (P <0.05). Conclusions Ligation of different coronary arteries has a direct impact on animal survival rate and model success rate in establishing aneurysm model. Ligation of the left anterior descending branch and left circumflex artery to establish the model of the aneurysm model has a high success rate, which can form a relatively fixed volume chamber Wall tumor.