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目的系统研究红树植物老鼠簕Acanthus ilicifolius的化学成分。方法利用硅胶柱色谱、Sephadex LH-20凝胶色谱、HPLC高效液相色谱等方法分离纯化化合物,根据波谱数据和理化常数确定其结构。结果与结论从老鼠簕甲醇提取物的石油醚部位分离鉴定了9个化合物,分别是豆甾-4-烯-3-酮(1),豆甾-4,22-二烯-3-酮(2),麦角甾-4-烯-3-酮(3),豆甾-4-烯-3,6-二酮(4),豆甾-4,22-二烯-3,6-二酮(5),豆甾-4,22-二烯-6β-羟基-3-酮(6),6β-羟基-豆甾-4-烯-3-酮(7),3β-羟基-豆甾-5,22-二烯-7-酮(8),3β-羟基-豆甾-5-烯-7-酮(9)。其中,化合物4～8为首次从该植物中分离得到。体外细胞毒筛选实验表明,化合物2、4和9具有中等的细胞毒活性。
Objective To study the chemical composition of Acanthus ilicifolius, a mangrove mouse. Methods The compounds were isolated and purified by column chromatography on silica gel, Sephadex LH-20 gel chromatography and HPLC. Their structures were elucidated on the basis of spectral data and physico-chemical constants. RESULTS AND CONCLUSION Nine compounds were isolated and identified from the petroleum ether fraction of the methanolic extract of S. argentris. They were stigmasterol-4-en-3-one (1), stigmasteroid-4,22- 2), ergosta-4-en-3-one (3), stigmast-4-ene-3,6-dione (4), stigmaster-4,22- (6), 6β-hydroxy-stigmaster-4-en-3-one (7), 3β-hydroxy-stigmaster- 5,22-dien-7-one (8), 3β-hydroxy-stigmaster-5-en-7-one (9). Among them, compounds 4 ~ 8 were isolated from this plant for the first time. In vitro cytotoxicity screening experiments showed that compounds 2, 4 and 9 have moderate cytotoxic activity.