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目的:通过高效液相色谱-柱后修饰荧光检测法测定大鼠血浆中葛根素和大豆苷元的含量,考察通脉方其他中药对葛根素和大豆苷元药动学行为的影响。方法:研究大鼠口服葛根提取液、葛根-丹参提取液、葛根-川芎提取液及葛根-川芎-丹参提取液(通脉口服液)的药动学特征,探讨通脉复方的配伍机制。结果:丹参和川芎对葛根素和大豆苷元的吸收、分布具有不同的影响,而通脉口服液给药组中的葛根素和大豆苷元血药浓度最高、血浆清除率最低,其药动学行为的改变是丹参、川芎综合作用的结果。结论:本方法灵敏、准确、快速、简便,从药动学角度初步探讨了通脉方中各组方药材配伍协同作用的机制。
OBJECTIVE: To determine the content of puerarin and daidzein in rat plasma by high performance liquid chromatography-post-column modified fluorescence detection and investigate the effect of other traditional Chinese medicines of Tongmai on the pharmacokinetics of puerarin and daidzein. Methods: The pharmacokinetics of Radix Puerariae, Radix Puerariae - Radix Salviae Miltiorrhizae, Radix Puerariae - Chuanxiong Rhizome and Radix Puerariae - Chuanxiong - Salvia Miltiorrhiza (Tongmai Oral Liquid) were studied, and the compatibility mechanism of Tongmai Formula was studied. Results: Radix Salviae Miltiorrhizae and Chuanxiong rhizome had different effects on the absorption and distribution of puerarin and daidzein. Puerarin and daidzein in Tongmai Oral Liquid group had the highest plasma concentration and the lowest plasma clearance rate. Changes in school behavior is the result of the comprehensive effect of Salvia, Chuanxiong. Conclusion: The method is sensitive, accurate, rapid and simple. The mechanism of synergic effect of different prescription herbs in Tongmai Fang was preliminarily discussed from the perspective of pharmacokinetics.