【摘 要】
：
A cholesterol oxidase(COD)was hybridized with Ca2+,Zn2+,Al3+,Fe2+and Mn2+.After precipitation with PO43-at 4℃for 72 h,the resulting pellets were freeze-dried.I
【机 构】
：
Key Laboratory of Industry Biotechnology(Ministry of Education),School of Biotechnology,Jiangnan Uni
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A cholesterol oxidase(COD)was hybridized with Ca2+,Zn2+,Al3+,Fe2+and Mn2+.After precipitation with PO43-at 4℃for 72 h,the resulting pellets were freeze-dried.In scanning electron microscopy assays,the metal-COD complexes revealed flower-like or granular structures after hybridization.Fourier transform infrared spectroscopy assay revealed the characteristic peaks of both the enzyme and metal materials.X-ray diffraction analysis indicated that COD was encapsulated in CaHPO4·2H2O-,Zn3(PO4)2·4H2O-,AlPO4-,FeP4-and Mn3(PO4)2·3H2O-based nanostructures,respectively.Differential scanning calorimetry assay indicated signifi-cant increases in thermo-denaturation temperatures from 60.5℃to 167.02℃,167.02℃,137.70℃,172.85℃and 160.99℃,respectively.Using steroid derivatives as substrates,this enzyme could convert cholesterol,pregne-nolone,dehydroepiandrosterone,ergosterol,β-sitosterol and stigmasterol to related single products.Hybridization in metal-based nanostructures could significantly enhance the initial conversion ratio and reaction stability of the enzyme.In addition,substrate selectivity could be affected by various metal materials.Briefly,using Ca2+,Zn2+,Al3+,Fe2+and Mn2+as hybrid raw materials could help to encapsulate COD in related metal-enzyme nanostructures,and could help to promote the stability and tolerant properties of the enzyme,while also enhancing its catalytic characteristics.
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