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目的研究二十二碳六烯酸(DHA)复合物的抗癌机制。方法以体外细胞培养的方法评价DHA复合物对U251胶质瘤细胞的抑制作用;以RT-PCR方法研究DHA复合物对U251胶质瘤细胞中Bcl-2和Bax mRNA含量的影响。结果DHA复合物U251神经胶质瘤细胞的IC50为0.3746μg/ml(0.3324～0.4168μg/ml);与阴性对照组比较,DHA复合物使U251胶质瘤细胞内Bcl-2 mRNA含量明显降低(P<0.01);Bax mRNA含量明显升高(P<0.01)。结论在本试验条件下,DHA复合物在体外对U251神经胶质瘤细胞有抑制作用,其机制可能为降低Bcl-2基因转录,增加Bax基因转录,从而通过促进细胞凋亡达到抗癌目的。
Objective To study the anticancer mechanism of docosahexaenoic acid (DHA) complex. Methods The inhibitory effect of DHA complex on U251 glioma cells was evaluated by cell culture in vitro. The effects of DHA complex on the expression of Bcl-2 and Bax mRNA in U251 glioma cells were studied by RT-PCR. Results The IC50 of DHA complex U251 glioma cells was 0.3746μg / ml (0.3324-0.4168μg / ml). Compared with the negative control group, DHA complex significantly decreased the content of Bcl-2 mRNA in U251 glioma cells P <0.01). The content of Bax mRNA increased significantly (P <0.01). Conclusions Under the experimental conditions, DHA complex can inhibit U251 glioma cells in vitro. Its mechanism may be to reduce the transcription of Bcl-2 gene and increase the transcription of Bax gene, so as to achieve anticancer effect by promoting apoptosis.