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目的 探讨倒卵叶五加总皂甙 (SAOH)对大鼠缺血心肌再灌注后心肌顿抑的作用。方法 采用结扎左冠状动脉前降支 30 m in,再灌注复制大鼠心肌缺血 /再灌注损伤 (IRI)的模型 ,结扎前 10 min注射 SAOH,采用左心室插管 ,动态监测左心室心功能 ,并测定心肌再灌注 40 m in时心肌组织三磷腺苷酶 (ATPase)的活性和血浆一氧化氮(NO)。结果 IRI组、SAOH1 组 (5 0 mg/ kg)及 SAOH2 组 (10 0 m g/ kg)在缺血及再灌注过程中左心室收缩压和室内压上升或下降最大速率 (L VSP和± d P/ dtmax)均呈进行性下降 ,但 SAOH组下降趋势较缓。 SAOH组在整个再灌注过程中 ,L VSP和± d P/ dtmax均较 IRI组明显提高 ,甚至 SAOH2 组的 +d P/ dtmax值在再灌注即刻、再灌注 10 m in时即高于假手术组 (P均 <0 .0 1) ,再灌注 2 0 m in、30 min时与假手术组无明显差异。再灌注 40 min时 ,SAOH1 组、SAOH2 组较 IRI组心肌肌膜 Na+- K+- ATPase、Ca2 +- ATPase、Mg2 +- ATPase活性和血浆 NO水平明显升高 (P均<0 .0 1)。结论 SAOH对心肌顿抑有明显的改善作用 ,其机制之一可能与其提高心肌组织 ATPase活性和血浆NO含量 ,减少 Ca2 +超载有关。
Objective To investigate the effect of total saponin (SAOH) of oblonging in vitro on myocardial stunning induced by reperfusion of ischemic myocardium in rats. Methods The left anterior descending coronary artery was ligated for 30 min. After reperfusion, myocardial ischemia/reperfusion injury (IRI) was induced in rats. SAOH was injected 10 min before ligation and left ventricular catheterization was used to dynamically monitor left ventricular heart function. The activity of myocardial triphosphatase (ATPase) and plasma nitric oxide (NO) were measured at 40 min of myocardial reperfusion. Results The maximum rate of left ventricular systolic pressure and intraventricular pressure increased or decreased during ischemia and reperfusion in IRI, SAOH1 (50 mg/kg) and SAOH2 (100 mg/kg) groups (L VSP and ± d P (/dtmax) showed a progressive decline, but the SAOH group showed a decreasing trend. In the SAOH group, L VSP and ± d P/dtmax were significantly higher than those in the IRI group during the entire reperfusion course. Even the value of +d P/dtmax in the SAOH2 group was higher than the sham operation immediately after reperfusion and 10 m in reperfusion. There was no significant difference between the sham operation group and the sham operation group (P<0.01), 20 min reperfusion, and 30 min. At 40 min after reperfusion, Na+-K+-ATPase, Ca2+-ATPase, Mg2+-ATPase activity and plasma NO levels in the SAOH1 and SAOH2 groups were significantly higher than those in the IRI group (P<0.01). Conclusion SAOH has a significant effect on the improvement of myocardial stunning. One of the mechanisms may be related to the increase of myocardial ATPase activity, plasma NO, and Ca2 + overload.