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目的了解杭州市家蝇乙酰胆碱酯酶(AChE)活性,分析其与残杀威高抗的相关性。方法采用点滴法测定家蝇对残杀威的抗药性表型,利用比色法测定家蝇现场种群与敏感品系的AChE水解活性及家蝇现场种群的残存活性。结果家蝇现场种群的半数致死量(LD_(50))>400μg/只,抗性倍数>2 500倍;家蝇敏感品系和现场种群的AChE水解活性分别为(0.291±0.114)和(0.360±0.104)μmol/(mg·min),差异有统计学意义(t=2.692,P=0.009);现场种群对残杀威的不敏感个体在种群中的比例为38.89%。结论杭州市家蝇现场种群AChE的生化特性较敏感品系发生了较大改变,AChE水解活性升高,对残杀威的敏感度降低,提示其可能是导致杭州市家蝇现场种群对残杀威产生高抗的主要原因。
Objective To understand the AChE activity of house fly in Hangzhou, and analyze the correlation between AChE activity and methocarbamide resistance. Methods The drug resistance phenotypes of Musca domestica to propoxur were determined by spotting method. The AChE hydrolytic activity of Musca domestica and its susceptible strains and the residual activity of Musca domestica were determined by colorimetry. Results The LC50 (LD 50) of the live population was> 400 μg / body and the resistance multiple was> 2 500 times. The AChE hydrolytic activities of housefly susceptible and live populations were (0.291 ± 0.114) and (0.360 ± 0.104) μmol / (mg · min), the difference was statistically significant (t = 2.692, P = 0.009). The proportion of insecticide inhabiting population to the predatory population in the population was 38.89%. Conclusion The biochemical characteristics of AChE in housefly populations of Hangzhou City changed greatly compared with those of susceptible strains. The hydrolysis activity of AChE increased and the susceptibility to propoxurdin decreased, suggesting that it might cause the high population of housefly in Hangzhou. The main reason for resistance.