论文部分内容阅读
胶孢炭疽菌(Colletotrichum gloeosporioides)引起的柱花草炭疽病是热带牧草柱花草的主要病害,利用根癌农杆菌介导转化(Agrobacterium tumefaciens mediated transformation,ATMT)的T-DNA插入突变技术,可使炭疽病菌基因组上被插入部位的基因丧失功能,是研究柱花草炭疽病菌致病机理的重要方法。在先前优化根癌农杆菌介导柱花草炭疽病菌遗传转化体系的基础上,构建了4 616个转化子的突变体库,从中随机选取部分突变体,对其TDNA插入拷贝数、遗传稳定性、生长速度、产孢量、分生孢子萌发等进行分析。结果表明,所得转化子多为单拷贝,能稳定遗传。一些转化子的生长速度、产孢量、分生孢子萌发率与野生型相比有明显差异。
Colletotrichum gloeosporioides caused Colletotrichum (Colletotrichum gloeosporioides) is the main disease of tropical forage plants, using T-DNA insertion mutation technology Agrobacterium tumefaciens mediated transformation (ATMT), anthrax It is an important method to study the pathogenic mechanism of Colletotrichum gloeosporioides in the gene loss function of the inserted site on the germ genome. Based on the previous optimization of Agrobacterium tumefaciens-mediated genetic transformation system of Colletotrichum gloeosporioides, 4 616 transformant mutants were constructed, and some mutants were randomly selected. The number of TDNA insertions, genetic stability, Growth rate, sporulation, conidial germination and other analysis. The results showed that the resulting transformants were mostly single copy, stable inheritance. The growth rate, sporulation rate and conidial germination rate of some transformants were significantly different from those of wild type.