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目的探讨三氧化二砷(As2O3)对体外造血微环境中KG1a细胞粘附分子CD44和CD49d表达的影响。方法用正常人和白血病患者的骨髓基质细胞在体外模拟造血微环境,与KG1a细胞共培养。用流式细胞术检测不同条件下As2O3对KG1a细胞CD44和CD49d表达的影响。结果来源于正常人或白血病患者的骨髓基质细胞与KG1a细胞共培养都可明显增加KG1a细胞CD44、CD49d表达(P≤0.01),但两种不同来源的骨髓基质细胞上调KG1a细胞CD44、CD49d表达无明显差异(P>0.05);骨髓基质细胞与KG1a细胞共培养的时间对CD44和CD49d表达无影响(P>0.05)。在共培养体中,不同浓度As2O3均可下调KG1a细胞CD44、CD49d的表达。用1μmol/L的As2O3分别作用24h及48 h后,KG1a细胞表面CD44分别为(94.32±0.77)%和(88.97±1.74)%(P<0.05);CD49d分别为(41.12±0.37)%和(34.12±1.77)%(P<0.05),随时间延长表达下降。当As2O3浓度增高为2μmol/L时,CD44表达率由(99.08±0.29)%降至(85.6±0.88)%,CD49d表达率由(47.48±0.1)%降至(37.03±0.96)%(P<0.01)。结论 As2O3能降低体外造血微环境中KG1a细胞表面CD44、CD49d表达,并且呈时间剂量依赖性。
Objective To investigate the effect of arsenic trioxide (As2O3) on the expression of CD44 and CD49d on KG1a cells in hematopoietic microenvironment. Methods Bone marrow stromal cells from normal and leukemia patients were used to simulate hematopoietic microenvironment in vitro and co-cultured with KG1a cells. Flow cytometry was used to detect the effect of As2O3 on the expression of CD44 and CD49d in KG1a cells under different conditions. Results Bone marrow stromal cells from normal and leukemia patients could significantly increase the expression of CD44 and CD49d in KG1a cells (P≤0.01). However, upregulation of CD44 and CD49d expression in KG1a cells by two different origins of bone marrow stromal cells (P> 0.05). The time of co-culture of marrow stromal cells and KG1a cells had no effect on the expression of CD44 and CD49d (P> 0.05). In co-culture, different concentrations of As2O3 could down-regulate the expression of CD44 and CD49d in KG1a cells. After treated with 1μmol / L As2O3 for 24 h and 48 h, the CD44 expression on KG1a cells was (94.32 ± 0.77)% and (88.97 ± 1.74)%, respectively (P <0.05) 34.12 ± 1.77)% (P <0.05), and the expression decreased with time. When the concentration of As2O3 was increased to 2μmol / L, the expression of CD44 decreased from (99.08 ± 0.29)% to (85.6 ± 0.88)% and the expression of CD49d decreased from (47.48 ± 0.1)% to (37.03 ± 0.96)% 0.01). Conclusion As2O3 can reduce the expression of CD44 and CD49d on KG1a cells in hematopoietic microenvironment in vitro and in a dose-and time-dependent manner.