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目的:建立高效液相色谱-质谱联用法测定人血浆中氯雷他定浓度。方法:分析柱为Gemini C18(5μm,50 mm×2.0mm),流动相为0.2%甲酸水溶液-甲醇(33.5∶66.5,v/v),流速:0.2 mL.min-1,柱温:25℃。采用四级杆质谱检测器电喷雾离子化(ESI)源,检测方式为正离子多离子反应监测(SRM)。结果:线性范围为0.2～25.6μg.L-1,平均提取回收率68.7%,日间RSD小于13.78%,日内RSD小于6.16%。结论:该方法快速、灵敏、重现性好,可用于临床血药浓度监测和药动学研究。
Objective: To establish a method for the determination of loratadine in human plasma by high performance liquid chromatography-mass spectrometry. Methods: The analytical column was Gemini C18 (5 μm, 50 mm × 2.0 mm). The mobile phase consisted of 0.2% formic acid in methanol (33.5:66.5, v / v) . Quadrupole mass spectrometry electrospray ionization (ESI) source was used with positive ion multi-ion reaction monitoring (SRM). Results: The linear range was 0.2 ~ 25.6μg.L-1, the average recovery was 68.7%, the intraday RSD was less than 13.78%, and the intraday RSD was less than 6.16%. Conclusion: The method is rapid, sensitive and reproducible. It can be used to monitor clinical plasma concentration and pharmacokinetics.