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目的探讨ＡｐｏＥ基因多态性与冠心病、脑梗塞的相关关系。方法应用聚合酶链反应－限制性片段长度多态性分析结合ＤＮＡ直接银染技术，对正常群体、冠心病群体和脑梗塞群体ＡｐｏＥ基因第４外显子进行分析。结果经χ２检验，ＡｐｏＥ基因Ｅ３／Ｅ３基因型在对照组与冠心病组（Ｐ＞０．２５）和脑梗塞组（Ｐ＞０．１）之间没有显著性差异；经小样本ｕ检验，ＡｐｏＥ等位基因ε２在对照组与冠心病组（Ｐ＞０．２５）和脑梗塞组（Ｐ＞０．１）之间，等位基因ε４对照组与冠心病组（Ｐ＞０．１）和脑梗塞组（Ｐ＞０．２５）之间均无显著性差异。结论研究未发现ＡｐｏＥ基因多态性与冠心病和脑梗塞之间存在相关关系。
Objective To investigate the relationship between ApoE gene polymorphism and coronary heart disease and cerebral infarction. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with DNA direct silver staining was used to analyze exon 4 of ApoE gene in normal population, coronary heart disease group and cerebral infarction population. Results There was no significant difference of ApoE gene E3 / E3 genotype between CHD group (P> 0.25) and cerebral infarction group (P> 0.1) by χ2 test. After small sample u test, ApoE allele ε2 between control group and coronary heart disease group (P> 0.25) and cerebral infarction group (P> 0.1), allele ε4 control group and coronary heart disease group (P> 0.1) And cerebral infarction group (P> 0.25), no significant difference between. Conclusion The study found no correlation between ApoE gene polymorphism and coronary heart disease and cerebral infarction.