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以具有不同开花习性的9份芸薹种作物为试材,根据开花相关基因BrFLC2序列设计引物进行PCR扩增,发现9份材料均获得约1 400 bp的片段。通过克隆测序分析发现9份材料存在片段大小差异,因此开发了与此相关的BrFLC2的InDel标记。通过对来自8个不同栽培种群的146份自然群体材料的基因型检测与开花时间的相关性分析发现:开花时间早晚与BrFLC2的InDel的基因型显著相关(r=0.412,P<0.01)。Del基因型材料开花时间(平均71 d)明显比In基因型(平均109 d)早。研究证明该标记与开花时间表型显著相关,可以用来进行分子标记辅助选择育种。
Nine Brassica crops with different flowering habits were used as test materials. According to the sequence of BrFLC2 gene, primers were designed and PCR amplified. The results showed that about 9 400 bp fragments were obtained. Cloning and sequencing analysis showed that there were fragment size differences in nine materials. Therefore, InDel labeling of BrFLC2 was developed. According to the correlation analysis between genotypes and flowering time of 146 natural populations from 8 different cultivated populations, it was found that the flowering time was significantly correlated with the InDel genotype of BrFLC2 (r = 0.412, P <0.01). Del genotype flowering time (average 71 d) was significantly earlier than the In genotype (mean 109 days). Studies have shown that the marker is significantly associated with the flowering phenotype and can be used for molecular marker-assisted selection breeding.