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目的 :用RAPD技术检测非小细胞肺癌组织的遗传改变。方法 :选用40条含10个碱基的随机引物对16例非小细胞肺癌进行RAPD扩增 ,扩增产物在含溴化乙锭的1.5 %琼脂糖凝胶中电泳 ,紫外透视仪上观察照相。结果 :40条引物都能扩增出清晰的条带 ,其中22条引物产生的条带在肿瘤与其相应正常组织间无差异 ,表现为单态性 ;18条引物扩增出的DNA序列在肿瘤与其相应正常组间存在差异 ,表现为带的缺失、增加、移动和带强弱差异。结论 :用RAPD技术检测出肺癌组织中具较高水平的遗传改变 ,这种改变与肺癌的发生相关。
Objective: To detect the genetic changes of non-small cell lung cancer by RAPD technique. METHODS: Twenty-six non-small cell lung cancers were randomly amplified by RAPD using 40 random primers containing 10 bases. The amplified products were electrophoresed on a 1.5% agarose gel containing ethidium bromide and photographed on a UV fluoroscope . Results: All the 40 primers amplified clear bands. Among them, the bands produced by 22 primers showed no difference between the tumors and their corresponding normal tissues, showing monomorphism. The DNA sequences amplified by 18 primers showed no significant difference And its corresponding normal group there is a difference between the performance of the band loss, increase, movement and differences in the strength of the band. Conclusion: The RAPD technique detects a high level of genetic alterations in lung cancer, a change associated with lung cancer.