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目的分析马蹄香EST资源的SSR信息,为开发EST-SSR标记奠定基础。方法从GenBank中获得马蹄香EST序列,用Sequencher4.8软件进行序列拼接得到Uni-EST序列,用SciRoKo3.4软件对Uni-EST序列进行SSR扫描,分析EST-SSR的分布频率和重复基元的类型特征。结果共获得10274条马蹄香EST序列,通过预处理共得到全长为5.11×106bp的无冗余Uni-EST6643条。在这些序列中共搜索出1408个SSR位点,分布在1232条Uni-EST序列中,发生频率为18.55%,EST-SSR的平均长度为22.30bp,平均每3.63kb含1个SSR位点。单核苷酸重复在马蹄香EST-SSR中占主导地位,发生频率为12.24%,其次为二核苷酸重复,发生频率为5.01%。在所有重复基元中,A/T基元出现频率最高,其次为AG/CT。结论马蹄香EST中SSR出现的频率较高,并且类型较为丰富。
OBJECTIVE: To analyze the SSR information of EST resources of Horseshoe sambal and lay the foundation for the development of EST-SSR markers. Methods The EST sequences of Marigold was obtained from GenBank. The sequence of the ESTs was sequenced using Sequencher 4.8 software to get the Uni-EST sequences. The SSR of Uni-EST sequences was screened with SciRoKo 3.4 software to analyze the distribution frequency of EST-SSR and the number of repeat motifs Type characteristics. Results A total of 10274 EST sequences were obtained. The total length of the untreated Uni-EST6643 was 5.11 × 106bp. A total of 1408 SSR loci were found in these sequences, which were distributed in 1232 Uni-EST sequences, with a frequency of 18.55%. The average length of EST-SSR was 22.30bp, with an average of 1. SSR loci per 3.63kb. Mononucleotide repeats dominated EST-SSR, with a frequency of 12.24%, followed by dinucleotide repeats with a frequency of 5.01%. Among all the repeat motifs, A / T motifs appeared most frequently, followed by AG / CT. Conclusion The SSR frequency of horseshoe fragrant EST is higher and more abundant.