论文部分内容阅读
近年来已陆续识别出许多重要病毒的保护性蛋白质上的主要抗原部位.主要的识别方法有:(1)确定病毒抗原的三维结构;(2)比较自然变异抗原的氨基酸序列;(3)对单克隆中和抗体选择的病毒突变株进行核苷酸序列分析;(4)识别出病毒保护性抗原的高度亲水区,一般位于抗原表面和抗体可接近的部位.对病毒抗原氨基酸序列研究的迅速发展,推动了合成与病毒主要抗原部位相应的寡肽的研究,并设计出无感染性的病毒亚单位疫苗.亚单位疫苗能解决全病毒免疫原带来的一些问题,例如病毒灭活不全或减毒不全可能引起的疫苗的生物学污染.然而,在合理设计一种亚单位疫苗时,需要详细了解病毒
In recent years, one of the major antigenic sites on the protective proteins of many important viruses has been identified. The main identification methods include: (1) determining the three-dimensional structure of viral antigens; (2) comparing the amino acid sequences of naturally occurring antigenic antigens; (3) (4) Identify the highly hydrophilic region of the virus protective antigen, usually located on the surface of the antigen and accessible to the antibody.Research on the amino acid sequence of the virus antigen Rapid development, and promote the synthesis of the main antigenic site of the virus corresponding oligopeptide research and design of non-infectious virus subunit vaccine subunit vaccine can solve some of the problems caused by full virus immunogens, such as virus inactivation Or attenuated toxin may cause biological contamination of the vaccine However, in the rational design of a subunit vaccine, you need to learn more about the virus