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巳知在胚胎发育期脑内已存在阿片肽和阿片受体,其机能意义未明.鉴于大鼠出生后一段时期,脑发育仍在进行,本文在婴鼠生后连续注射纳洛酮(Nal)和甲硫氨酸脑啡肽(MEK)观察其对幼鼠吸乳迷津分辨学习(ADL)和Y迷津明暗分辨学习(BDL)和前脑蛋白含量的影响。 实验选用SD大鼠幼仔(n=62),生后随机分组,雌雄各半,生后第一天开始,每日颈部皮下分别注射Nal(10、50、100、200μg/100g B W),MEK(20μg/1O0gB W)或等量的生理盐水.连续注射14天。分别于16日龄和30日龄进行ADL和BDL行为训练。45日龄处死并测脑蛋白含量(紫外吸收法)。分别
It has been known that opioid receptors and opioid receptors are already present in the brain during embryonic development, and its function and significance are not clear. Since brain development is still underway for some time after birth, And methionine enkephalin (MEK) on the expression of ADL and BDL and the protein content of forebrain in young rats. In the experiment, SD rats (n = 62) were randomly divided into male and female rats. The first day after birth, Nal (10, 50, 100, 200μg / 100g BW) MEK (20μg / 1O0gB W) or the same amount of saline continuous injection for 14 days. ADL and BDL behavioral training were performed at 16 and 30 days of age respectively. Animals were sacrificed at 45 days of age and brain protein content was measured (UV absorbance). respectively