论文部分内容阅读
以 RP-HPLC测定了感冒退热冲剂中连翘酯苷A的含量;连翘酯苷A以冷浸超声法直接用甲醇提取;色谱条件为:ODS色谱柱(250mm×4.6 mm,5μm);甲醇(含1%的四氢呋喃)-水(含0.01 mol/L磷酸二氢钾,pH 3.2)为流动相进行梯度洗脱;柱温室温;检测波长 332 nm;实验表明本法简便、特异、准确,可用于本制剂的质量控制。
The content of Forsythiaside A in Ganmao Fuli Granules was determined by RP-HPLC. Forsythiaside A was directly extracted by methanol with cold soaking. The chromatographic conditions were: ODS column (250mm×4.6mm, 5μm). Methanol (containing 1% tetrahydrofuran)-water (containing 0.01 mol/L potassium dihydrogen phosphate, pH 3.2) as mobile phase gradient elution; column temperature at room temperature; detection wavelength 332 nm; The method is simple, specific and accurate and can be used for quality control of the preparation.