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目的:建立HPLC法测定人血浆中右酮洛芬的浓度。方法:血浆样品用乙醚作为萃取剂萃取,以萘普生为内标;采用DiamonsiLC18色谱柱(5μm,4.6 mm×200 mm);流动相为乙腈-5 mol/L磷酸盐缓冲液(27∶73),pH值为6.9,流速为1.0 mL/min;检测波长为260 nm。结果:右酮洛芬血浆样品的线性范围为0.078~5.000μg/mL(r=0.999 6);方法回收率和萃取回收率分别为99.5%~101.9%和70.8%~73.8%(n=5),日内、日间RSD分别为5.5%~10.8%和6.4%~9.4%(n=5)。结论:本法简便、准确、灵敏度高、专属性强,适用于人血浆中右酮洛芬浓度的测定。
Objective: To establish a HPLC method for the determination of dexketoprofen in human plasma. METHODS: Plasma samples were extracted with diethyl ether as extraction solvent and naproxen as internal standard. Diamonsi LC18 column (5 μm, 4.6 mm × 200 mm) was used. The mobile phase was acetonitrile-5 mol / L phosphate buffer (27:73 ), pH 6.9, flow rate 1.0 mL / min and detection wavelength 260 nm. Results: The linear range of dexketoprofen plasma was 0.078-5.000μg / mL (r = 0.999 6). The recoveries and recoveries were 99.5% -101.9% and 70.8% -73.8%, respectively (n = 5) Day and day RSD were 5.5% ~ 10.8% and 6.4% ~ 9.4% (n = 5) respectively. Conclusion: The method is simple, accurate, sensitive and specific. It is suitable for the determination of dexketoprofen in human plasma.