论文部分内容阅读
目的:通过观察P450arom、环氧化酶2(COX-2)、前列腺素E2(PGE2)在子宫腺肌病(AM)治疗前后随动情周期变化的表达差异,及其各组在位及异位内膜上的表达差异,研究内异康复片治疗AM的作用靶点,以探索内异康复片治疗AM的作用机制。方法:采用异体垂体移植的手术方法造模,采用免疫组化方法,观察各组在动情期和间情期及治疗前后在位及异位内膜雌激素效应相关因子的表达。结果:P450、PGE2、COX-2的表达,在6月模型组和3月模型组显著高于正常组(P<0.01),且6月模型组高于3月模型组(P<0.05)。3个指标的表达在不同治疗组治疗后有所下降(P<0.05,P<0.01),且内异康复片高剂量组优于其它各治疗组。动情期指标表达高于间情期(P<0.05)。结论:内异康复片能够通过下调在位及异位内膜雌激素效应相关因子P450、COX-2、PGE2表达,阻断雌激素生成的正反馈环,抑制雌激素对异位病灶的影响效应,从而控制和治疗AM,缓解临床症状。
Objective: To observe the changes of estrous cycle of P450arom, cyclooxygenase 2 (COX-2) and prostaglandin E2 (PGE2) before and after treatment of adenomyosis (AM) Intima of the expression differences in the study of the different effects of the therapeutic effect of AM within the target of AM to explore the mechanism of action of the Nei Yi Kang tablets treatment. Methods: Allogeneic pituitary grafting was used to establish the model. Immunohistochemistry was used to observe the expression of estrogen-related factors in eutopic and ectopic endometrium in estrus and estrus and before and after treatment. Results: The expressions of P450, PGE2 and COX-2 in the model group and the model group were significantly higher than those in the normal group (P <0.01) in June and June respectively. The expression of three indexes decreased in different treatment groups after treatment (P <0.05, P <0.01), and Neiyisi Tablet high-dose group was superior to other treatment groups. The expression of estrus indicators was higher than that of estrus (P <0.05). CONCLUSION: Nei Yi Kang Fu Chang can inhibit the effect of estrogen on ectopic lesion by down-regulating the expression of estrogen-related factors P450, COX-2 and PGE2 in the eutopic and ectopic endometrium and blocking the positive feedback loop of estrogen production , So as to control and cure AM, relieve clinical symptoms.