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Objective:To investigate the activity of the active principle in the spines of the seed pods of Mucuna pruriens using contraction of guinea pig ileum as index of pharmacological activity. Methods:The active principle was extracted with 0.0015 M NaCl.Muscle strips of guinea pig ileum were prepared and contractile responses were measured using a Kymograph.Two sets of experiment were conducted:(1).The contraction of the ileum in presence of different concentrations of histamine,2-methylhistamine and the extract of Mucuna pruriens.(2).The contractile response of the ileum in presence of different concentrations of the extract and antagonists including diphenhydramine,atropine and methysergide.Results:(1) The extract of Mucuna pruriens hair, 2- methylhistamine and histamine produced dose dependent contraction of guinea pig ileum (Extract ED_(50) = 13.0μg/mL,2-methylhistamine ED_(50)=8.5μg/mL and histamine ED_(50)=10.0μg/ mL).(2) Diphenhydramine,an H,antagonist competitively blocked the contractile response of the Mucuna pruriens extract(3) Coadminstration of the Mucuna pruriens extract either with different doses of antimuscarinic agent atropine or 5-hydroxytryptamine blocking agent methysergide did not alter the extract induced contractile response of the guinea pig ileum.Conclusion:These results demonstrate that the spines of Mucuna pruriens possess histamine activity which may contribute to its itching and painful irritation effects.
Objective: To investigate the activity of the active principle in the spines of the seed pods of Mucuna pruriens using contraction of guinea pig ileum as index of pharmacological activity. Methods: The active principle was extracted with 0.0015 M NaCl. Muscle strips of guinea pig ileum were prepared and contractile responses were measured using a Kymograph. Two sets of experiments were conducted: (1). The contraction of the ileum in presence of different concentrations of histamine, 2-methylhistamine and the extract of Mucuna pruriens. (2). contractile response of the ileum in presence of different concentrations of the extract and antagonists including diphenhydramine, atropine and methysergide. Results: (1) The extract of Mucuna pruriens hair, 2-methylhistamine and histamine produced dose dependent contraction of guinea pig ileum (Extract ED (50) = 13.0 μg / mL, 2-methylhistamine ED 50 = 8.5 μg / mL and histamine ED 50 = 10.0 μg / mL.) Diphenhydramine, an H, antagonist competitively blocked the c ontractile response of the Mucuna pruriens extract (3) Coadminstration of the Mucuna pruriens extract either with different doses of antimuscarinic agent atropine or 5-hydroxytryptamine blocking agent methysergide did not alter the extract induced contractile response of the guinea pig ileum. Confclusion: that the spines of Mucuna pruriens posse histamine activity which may contribute to its itching and painful irritation effects.