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为了研究脑缺血时兴奋性氨基酸与胆碱能神经的关系，采用双侧颈总动脉夹闭（ＣＣＡＯ）的脑缺血动物模型，用乙酰胆碱离子选择性微电极（ＡＣｈ－ＩＳＭｓ）检测皮层ＡＣｈ释放量，观察脑缺血再灌注时谷氨酸对大鼠皮层ＡＣｈ释放量的影响。结果表明：１０－１ｍｏｌ／ＬＧｌｕ对ＡＣｈ－ＩＳＭｓ无干扰作用，在生理状态下不能显著地促进皮层ＡＣｈ的释放，但可使脑缺血３ｍｉｎ时皮层ＡＣｈ释放量较未加Ｇｌｕ组增加５８．１％（Ｐ＜０．０１），再灌注后皮层ＡＣｈ活性恢复减慢。结果提示：Ｇｌｕ协同ＡＣｈ释放的效应在脑缺血时明显放大，推测Ｇｌｕ和ＡＣｈ可能在缺血性脑损伤中有放大的协同作用。
In order to study the relationship between excitatory amino acids and cholinergic nerves in cerebral ischemia, the model of cerebral ischemia in bilateral common carotid artery occlusion (CCAO) was established. ACh-ISMs were used to detect the expression of ACh Release, to observe the effect of glutamate on ACh release from cerebral cortex in cerebral ischemia-reperfusion rats. The results showed that: 10-1mol / LGlu had no interference with ACh-ISMs, and could not significantly promote the release of ACh in the cortex under physiological conditions, but the ACh release in the cortex was increased by 58.1 % (P <0.01), and the ACh activity in the cortex recovered slowly after reperfusion. The results suggest that: Glu synergistic effect of ACh release in cerebral ischemia was significantly enlarged, speculated that Glu and ACh may have synergistic effects in ischemic brain injury synergies.